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Science Journal

 

Report and Opinion

Volume 3 - Issue 1, Cumulated 19, January 1, 2011, ISSN 1553-9873

Cover Page, Introduction, Contents, Call for Papers, Report0301

 

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CONTENTS

 No.

Titles / Authors

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1

The diagnostic value of faecal calprotectin in differentiating inflammatory bowel diseases (IBD) from irriable bowel syndrome (IBS)

 

Hesham Ezz El Din Said1, Ahmed Aly Monis2, Manal Mohammed Abd El Aziz3, Engy Yousry El Sayed4, Sherif Sadek Shabana5, Ahmed Samir Abd El Sadek6

1,2,4,5,6Intenal Medicine Departement, Ain Shams University, 3Clinical Pathology Departement, Ain Shams University

ashorengy@yahoo.com

 

 Abstract: Background: Patients with inflammatory bowel disease and irritable bowel syndrome can have overlapping symptoms, yet a different management. Hence, a noninvasive biological marker is needed for the assessment of patients with lower bowel symptoms. Aim: This study aimed at evaluating the the diagnostic value of faecal calprotectin as a potential marker in differentiating patients with inflammatory bowel disease from those with a irritable bowel syndrome. Methods: twenty patients with IBD and twenty patients with IBS were recruited from Ain shams university outpatient clinic in the period between January 2008 to November 2009. In addition, a control group of 10 healthy individuals was included. Faecal calprotectin level using an ELISA technique (Calprest®) was measured in the stool of all groups. Also, atypical p-ANCA and ASCA were performed in the IBD group. Results: At a cut off value of 8.1 mg/L, fecal calprotectin had a negative predictive value (NPV) of 100% to exclude IBS patients with a sensitivity of 100% and a positive predictive value (PPV) to confirm IBD of 95.24% with a specificity of 95%. The diagnostic accuracy of faecal calprotectin in predicting IBD activity was 100% at a cut off value of 25.5 mg/L. Conclusion: fecal calprotectin appears to be a clinically useful noninvasive marker in differentiating IBD from IBS.

[Hesham Ezz El Din Said, Ahmed Aly Monis, Manal Mohammed Abd El Aziz, Engy Yousry El Sayed, Sherif Sadek Shabana, Ahmed Samir Abd El Sadek. The diagnostic value of faecal calprotectin in differentiating inflammatory bowel diseases (IBD) from irriable bowel syndrome (IBS). Report and Opinion 2011;3(1):1-8]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.01

 

Keywords: Inflammatory bowel disease, irritable bowel syndrome, faecal calprotectin

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Biological Invasions, Its Type And Impact On Global Scale: A Review

 

1Kuldip S. Dogra, 2Ravinder K. Kohli, 1Sarvesh K. Sood, 2Parveen K. Dobhal and 1Seema Sharma

1Department of Biosciences, Himachal Pradesh University, Summer Hill, Shimla-171005, India

2Department of Botany, Panjab University Chandigarh- 160014, India

E.mail: dograks6@yahoo.co.in; Mobile: 919816740648; 919459170683

 

Abstract: Biological invasion is now recognized world over and causing loss of indigenous wealth on earth. Biological invasions are altering ecosystem functions by reducing indigenous species diversity, altering soil dynamics and ecology. Economic losses due to invasion process found to be huge and difficult to measure. If this process of biological invasion is remain continuous for years to come than we can only transfer monocultures of species to our future generations. To preserve our indigenous species diversity it is important to understand the biological invasion its type and impact on global scale. In view of this present paper presents an insight into the biological invasion, its type and impact.

[Kuldip S. Dogra, Ravinder K. Kohli, Sarvesh K. Sood, Parveen K. Dobhal and Seema Sharma. Biological Invasions, Its Type And Impact On Global Scale: A Review. Report and Opinion 2011;3(1):9-20]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.02

 

Keywords: Alien species, Biological Invasion, Biodiversity, Ecosystems, Invasive species

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Antibiotic Resistant Bacillus cereus in Some Selected Foods in Osun State, Nigeria.

 

*1Olawale, Adetunji, Kolawole and 2Akintobi, Akinsoji, Olubiyi

1Department of Applied Sciences, Osun State Polytechnic, Iree. Nigeria.

2Department of Biochemistry and Microbiology, Lead City University, Ibadan. Nigeria.

E-mail: akolawale@yahoo.com

 

Abstract: Microbiological analyses were carried out on Four hundred samples of five different foods i.e. boiled maize, cooked rice, garden egg, carrot and roasted beef from four towns Osogbo, Ilesha, Ife and Ikirun in Osun State, Nigeria. Food samples were collected from different vendors and examined for B. cereus, using B. cereus selective Agar Base, egg yolk emulsion and Bacillus selective supplement. B. cereus was detected in 202 (50.50%) of 400 food samples. B. cereus occurred in 71% of boiled maize, 63.75% cooked rice, 51.25% roasted beef (suya), 40% carrot and 23.75% garden egg. The range of population of B. cereus in these foods was 1.3 x 102 to 7.5 x 105 cfu/g. Boiled maize and cooked rice had highest mean counts of 105 cells/g. The level of B. cereus contamination of foods examined was higher in Ikirun compare to other towns in the state. From the results of antibiotic susceptibility test, almost all the Bacillus cereus strains were susceptible to gentamicin (98.24%) chloramphenicol (96.70%), and sulphamethoxazole (92.92%). The highest level of antibiotic resistance was obtained for ampicilin (70.80%), and amoxicillin (42.23%). The incidence of B. cereus foodborne intoxication could be reduced by maintaining high level of hygiene in our environment and good production practices.

[Olawale, Adetunji, Kolawole and Akintobi, Akinsoji, Olubiyi. Antibiotic Resistant Bacillus cereus in Some Selected Foods in Osun State, Nigeria. Report and Opinion 2011;3(1):21-25]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.03

Keywords: Bacillus cereus, Antibiotic resistant, Food intoxication, Hygiene, Food contamination

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Comparative study on the protective effect of Biphenyl Dimethyl Dicarboxylate (DDB) and Silymarin in Hepatitis induced by carbon tetrachloride (CCl4) in rats.

Hoda. A. Megahed*, Hanan G. Zahran *, Mahmoud. S. Arbid*, A. Osman** and Soha M.Kandil***

National Research Centre, ** Vet. Medicine C.U. and *** Kaser El Ainy new Hospitals C.U (C.U = Cairo University), City, Country

drhodamegahed@yahoo.com

Abstract: Study on the possible protective effect of (DDB) and Silymarin on Hepatitis induced by CCl4 was carried out. Injection of CCl4 daily orally administered to rats in a dose of 2.5ml/kg for three days significantly increase the activity of AST, ALT, ALK. Ph. Bilirubin and GGT by several folds of increase, also urea and creatinin were elevated by CCl4 given orally. Administration of DDB and Silymarin orally seven day after administration of CCl4 for three days Significantly decrease liver and kidney enzyme DDB and Silymarin administered before CCl4 to rats also significantly decrease the activity of liver and kidney enzymes. Histopathological investigation of this study show good confirmation to biochemical analysis.

[Hoda. A. Megahed, Hanan G. Zahran, Mahmoud. S. Arbid, A. Osman and Soha M.Kandil. Comparative study on the protective effect of Biphenyl Dimethyl Dicarboxylate (DDB) and Silymarin in Hepatitis induced by carbon tetrachloride (CCl4) in rats. Report and Opinion 2011;3(1):26-36]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.04

Keywords: Hepatitis, DDB, Silymarin, rats

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A Linear Programming Model For Short Sea Shipping And Multimodal Inland Transportation In Myanmar

 

Mu Mu Han, Lin Guolong, Yang Bin

Logistics Research Center, Shanghai Maritime University, Shanghai 200135, China

mumuhan@gmail.com

 

ABSTRACT: This paper considers the problem of determining transportation quantity and mode in transporting international cargoes between Myanmar and her trading countries, especially focusing on the countries in South East Asia to check the extent of using short sea shipping, and inland transportation. The objective of the paper is to minimize transportation costs by mode between cargo origin and destination, subject to the maximum cargo volumes being handled at each seaport. In order to optimize the short sea shipping and inland transportation in Myanmar, this paper suggests a linear programming model, which is an operations research technique.

[Mu Mu Han, Lin Guolong, Yang Bin. A Linear Programming Model For Short Sea Shipping And Multimodal Inland Transportation In Myanmar. Report and Opinion 2011;3(1):37-43]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.05

 

Keywords-Myanmar, Short sea shipping, Multimodal transportation, Linear programming model, Container cargo

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Effect Of Crude Ethanol Extract Of Nauclea Latifolia On Some Clinical Isolates Of Food Importance And Its Toxicological Potentials

 

Ogueke, C.C. 1, Chikwendu C.I. 2, Iwouno, J. O.1 And Ogbulie, J. N.2

Department of Food Science Technology, Federal University of Technology Owerri, Nigeria. PMB. 1526 Owerri, Nigeria.

Department of Microbiology, Federal University of Technology Owerri, Nigeria. PMB. 1526 Owerri, Nigeria.

 

Abstract: The leaves of Nauclea latifolia used in traditional medicine for the treatment of diarrhea were extracted in hot and cold ethanol with the aim of determining their antibacterial activities and toxicological potentials. Soxhlet extraction method was used for the hot ethanol extraction while for the cold ethanol ground samples were soaked in ethanol for 48h. The Agar diffusion method was used for the antibacterial assay at different concentrations on Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi. Albino rats were used for toxicological studies by injecting varying doses of the extracts through the intraperitoneal route for 14 days. The growth of S. typhi was not inhibited by the extract. However the hot ethanol extract had minimum inhibitory concentrations (MICs) of 3.24mg/ml, 3.28mg/ml and 4.82mg/ml on E. coli, S aureus and P. aeruginosa respectively, while the cold ethanol extract produced MICs of 4.74mg/ml, 5.14mg/ml and 5.61 mg/ml respectively on the isolates. Hematological analyses revealed that RBC, PCV and Hb values decreased with increase in doses of extract while ESR and WBC values increased. MCHC values were lower than that obtained from the control. However, statistical analyses revealed that there were significant differences in the values obtained for RBC, PCV and Hb at the lower doses and the controls. The extracts contained alkaloids, tannins and saponins. The antibacterial assay justifies the use of the plant in palm wine preservation and possible use in preservation of other foods, and the treatment of diarrhoea.

[Ogueke, C.C., Chikwendu C.I., Iwouno, J. O. And Ogbulie, J. N. Effect Of Crude Ethanol Extract Of Nauclea Latifolia On Some Clinical Isolates Of Food Importance And Its Toxicological Potentials. Report and Opinion 2011;3(1):44-52]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.06

 

Key words: Antibacterial; toxicological; preservation; ethanol extracts; Nauclea latifolia; clinical isolates

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Antibacterial Properties Of The Green Alga Pithophora Oedogonia (Mont.) Wittrock

 

*Pamela Sukumaran and **Thevanathan, R

*Department of Botany, Women’s Christian College, Chennai 600 006. Tamilnadu India

pamelasukumaran@yahoo.com

**Professor CAS in Botany, University of Madras

Guindy campus,Chennai 600 025. Tamilnadu India

 

ABSTRACT: Methanol and n-hexane extracts of the green alga, Pithophora oedogonia was tested for antibacterial activity against clinical isolates of common human pathogenic bacteria namely, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Salmonella typhi, Pseudomonas aeruginosa, Vibrio cholerae, Shigella flexnerii, Staphylococcus aureus, Streptococcus pyogenes and Streptococcus faecalis. Methanolic extract residue dissolved in diethylether exhibited good activity against Streptococcus pyogenes, Streptococcus faecalis and Escherichia coli. Activity of silica gel column fractions is significant and comparable to that of standard antibiotics. Chromatatron fractions recorded very low MIC values for Streptococcus pyogenes, Streptococcus faecalis and Escherichia coli as compared to that of standard antibiotics. The findings presented in this paper suggest that the ‘nuisance alga’. Pithophora oedogonia, could serve as a potential source of biologically active natural products for pharmaceutical application.

[Pamela Sukumaran and Thevanathan, R. Antibacterial Properties Of The Green Alga Pithophora Oedogonia (Mont.) Wittrock. Report and Opinion 2011;3(1):53-60]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.07

 

Keywords: Antibacterial; Green Alga; Pithophora Oedogonia (Mont.); Wittrock

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Weed flora infesting saffron (Crocus sativus L.) fields of Pampore, kashmir

 

*Bilal Ahmad Wani, and Nisar Ahmad Wani

Department of Botany University of Kashmir, Hazratbal Srinagar –190006 (India)

*Corresponding author Email :- arfee.b@rediffmail.com,

Woodscience.2005@gmail.com

 

Abstract: Saffron production is limited by a number of factors, among which weed infestation is prime component as these compete for space, light, water and nutrient therefore, reducing qualitative and quantitative yields of crop. The survey was conducted during 2005-2009 in different saffron fields of Pampore, Kashmir in order to know about the weeds infesting these fields. During the survey 88 species of weeds were identified which were annual, biennial and perennial in nature.

[Bilal Ahmad Wani, and Nisar Ahmad Wani. Weed flora infesting saffron (Crocus sativus L.) fields of Pampore, kashmir. Report and Opinion 2011;3(1):61-64]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.08

 

Key words:- Pampore, Saffron, Weeds, Identification.

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Immunohistochemical Study Of Protein P53 In Egyptian Psoriasis

 

Rasha EL-Adel; Mahmoud Abdel Hameed;Marwa El-Shaer*; Adel Imam **;Noha Abdel Hafez*

Dermatology and Venereology Department, Medical Research Division, National Research Centre ,Cairo Egypt

*Pathology Department , Medical Research Division, National Research Centre ,Cairo Egypt

** Dermatology and Venereology Department, Ain Shams University,Cairo Egypt

Corresponding Author:marwaelshaer@hotmail.com

12311 ,Elbuhouth Street ,Dokky,Cairo .Egypt

Abstract: Background: The histopathologic changes characteristic of psoriasis might be related to an abnormality in the apoptotic pathway. Aim of the work:The aim of this study is to evaluate the possible role of protein P53 in the pathogenesis of psoriasis through a case control study as it could be one of the targets of psoriasis therapy. Patients and Methods: This study included; 30 patients of different clinical variants of psoriasis and 25 controls normal skin biopsies. All patients were subjected to complete history taking, clinical examination including psoriasis area and severity index (PASI) score and skin biopsies, all patients stopped topical or systemic medication 4 weeks prior to biopsies.Five mm incisional biopsy specimens were taken from the 30 patients and from each biopsy one stained with hematoxylin and eosin to confirm the diagnosis ,the other to be prepared for immunohistochemical detection using mouse monoclonal antibody (Do7) against P53 protein , results were compared with 25 control ..Results: Psoriatic plaques revealed P53 nuclear staining detected in 13 out of the 30 patients (43.3%), and 17 (56.7%) showed negative immunoreactivity in keratinocytes. Conclusion: From these results it can be concluded that apoptosis plays a role in the pathogenesis of psoriasis and this may be mediated through abnornal expression of apoptosis regulating proteins P53.

[Rasha EL-Adel; Mahmoud Abdel Hameed; Marwa El-Shaer; Adel Imam; Noha Abdel Hafez. Immunohistochemical Study Of Protein P53 In Egyptian Psoriasis. Report and Opinion 2011;3(1):65-84]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.09

Key words: Apoptosis , immunohistochemistry, P53, Psoriasis

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Economic Efficiency Of Leafy Vegetable Production In Oyo State, Nigeria

 

Ogunniyi, L.T.

*Department Of Agricultural Economics And Extension, Ladoke Akintola University Of Technology Ogbomoso

E- mail: titiogunniyi@yahoo.com

 

ABSTRACT: The economic efficiency of leafy vegetable production in Oyo State, Nigeria was investigated using stochastic frontier production function, which incorporates a model f inefficiency effects. Primary data were collected through the use of structured questionnaires from 120 leafy vegetable farmers randomly selected from four local government areas. Stochastic frontier production function, using maximum likelihood estimation (MLE) was used to analyze the economic efficiency. The MLE results revel that farm size, family labour, hired labour, seed and fertilizer are the major factors influencing gross margin in leafy vegetable production. The efficiency model shows that older farmers and farmers with more extension contacts tend to be less economically efficient in leafy vegetable production. The mean economic efficiency score is 0.42.

[Ogunniyi, L.T. Economic Efficiency Of Leafy Vegetable Production In Oyo State, Nigeria. Report and Opinion 2011;3(1):85-91]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.10

 

Keywords: leafy vegetable; stochastic frontier model; economic efficiency

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Protective Effects of Bixa orellana Seed Oil on Carbon tetrachloride Induced Liver Damage in Rats

 

Wilson Obidah*, Grace K. Garba, Joseph Z. Fate#

Department of Biochemistry, Federal University of Technology Yola P.M.B.2076 Yola, Nigeria.

#Department of Science Laboratory Technology, Federal Polytechnic Mubi P.M.B. 35 Mubi, Nigeria.

domwam@yahoo.com

 

Abstract: The hepatoprotective effects of B.orellana seed oil on CCl4-induced liver damage were determined in male Wister strain rats (150±10g). Rats were pretreated with B.orellana seed oil at 0, 1, 5 or 10% (w/w) through dietary exposure for 4 weeks before a single intrapretoneal injection of CCl4. Serum biochemical parameters, liver lipid peroxidation and relative organ weights were determined. Pretreatment with B.orellana seed oil (10%) resulted in significant (p<0.05) reduction in serum liver enzymes markers activities, total bilirubin concentration, lipid peroxidation and relative liver weights induced by CCl4 administration. These results show that dietary exposure to B. orellana seed oil exhibited moderate protection against CCl4 induced hepatotoxicity in rats.

[Wilson Obidah, Grace K. Garba, Joseph Z. Fate. Protective Effects of Bixa orellana Seed Oil on Carbon tetrachloride Induced Liver Damage in Rats. Report and Opinion 2011;3(1):92-95]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.11

 

Key words: Bixa orellana; seed oil; hepatoprotection; CCl4; rat

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Antimicrobial potentials of some spices on beef sold in Gwagwalada market, FCT, Abuja

 

 

Agarry Olubunmi Olaitan*, Ugoh Sylvanus Chukwudi and Abeku Margaret

Department of Biological Sciences, University of Abuja, Nigeria

Corresponding author: oluagarry@yahoo.com

 

ABSTRACT: Studies on the antimicrobial activities of some spices on beef sold in Gwagwalada market, F.C.T, Abuja were carried out. The spices were chopped to sizes and were mixed with the beef sample. The bacterial load count of the beef sample before treatment is 1.9 x 107 and after treatment were 1.0x103, 1.5 x103 and 1.6x103 for garlic, thyme and bayleaf respectively. The fungal spore count of beef sample before treatment is 1.0x102 and x0.5102, 0.1x 102, 0.6x102 for the thyme, bayleaf and garlic respectively. The fungal spore count for the beef and sample after treatment with the spices combined is 0.1x101. The microbial isolates of beef sample include: Staphylococcus aureus, Pseudomonas spp, Proteus spp and Bacillus spp for bacterial isolates and fungal isolates were Aspergillus niger, Mucor spp, Rhizopus spp and Aspergillus flavus the combined effect of the three spices inhibited the growth of S. aureus, Bacillus spp, Proteus spp, A. flavus and Mucor spp.

[Agarry Olubunmi Olaitan, Ugoh Sylvanus Chukwudi and Abeku Margaret. Antimicrobial potentials of some spices on beef sold in Gwagwalada market, FCT, Abuja. Report and Opinion 2011;3(1):96-98]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.12

 

Keywords: Antimicrobial agents, spices, meat

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Comparative Effects Of Post Mortem Addition Of Natural And Synthetic Antioxidant Sources On Cooking Yield, Cooking Loss And Oxidative Stability Of Broiler Chichen Meat.

 

M. A. AYOOLA (PhD) 1, A .O. OLORUNSANYA (PhD) 2 AND A.O ADEDEJI 3

1. Department of Agricultural Science, Adeyemi college of Education, Ondo

2. Department of Animal Production, University of Ilorin, Ilorin

3. Department of Home Economics, Adeyemi college of Education, Ondo

(mayoola1@yahoo.co.uk)

 

Abstract: A worldwide concern in the use of synthetic antioxidants in food safety necessitates investigation into natural sources of antioxidants. An invitro assay of antioxidant properties of alpha-tocopheryl acetate, thyme leaf extract, Ethiopian pepper extract and wheat germ vitamin E was carried out using eight 14 weeks old broiler chicken. The muscle tissue of each chicken were separated, chopped, mixed thoroughly and divided into treatment group of 800g each. Experimental samples were prepared by blending 0.8% w/w addition of thyme leaf extract, Ethiopian pepper extract, wheat germ vitamin E and alpha-tocopheryl acetate while the control did not contain any. Each treatment sample was further divided into 8 sub-samples (80g each). Four samples per each treatment were separately packed in aluminum foil paper and refrigerated for 12 days at 4oC for raw meat Thiobarbituric Acid Reactive Substances (TBARS) analysis at interval of 3, 6, 9, and 12 days. Four sample per each treatment were steam cooked for 12 minutes, separately packed in aluminum foil paper and refrigerated for 6 days for cooked meat TBARS analysis at interval of 2, 4 and 6 days. The result indicated that post mortem addition of natural and synthetic sources of antioxidant had no significant effect (P>0.05) on cooking yield and cooking loss of broiler meat during storage. Comparatively, the tested natural antioxidant sources reduced the formation of peroxide more effectively (P<0.05) than alpha-tocopheryl acatete in refrigerated raw and cooked broiler chicken meat and could be used to replace synthetic compounds which poses health hazard to the consumers. However more studies are required to remove the chlorophyllization effect of thyme leaf extract and Ethiopian pepper extract on the meat.

[M. A. AYOOLA, A .O. OLORUNSANYA, A.O ADEDEJI. Comparative Effects Of Post Mortem Addition Of Natural And Synthetic Antioxidant Sources On Cooking Yield, Cooking Loss And Oxidative Stability Of Broiler Chichen Meat. Report and Opinion 2011;3(1):99-103]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.13

 

Key words: Comparative Effect; Mortem Addition; Antioxidant; Cook; Chichen Meat; Alpha-tocophery acetate, Ethiopian pepper, Thyme, lipid oxidation, rancidity

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[Report and Opinion 2011;3(1):104-119]. (ISSN: 1553-9873). http://www.sciencepub.net. 6

doi:10.7537/marsroj030111.14

Withdrawn

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Efficacy of antimicrobial effect of Venonia amygdalina and Tridax procumbens in in vitro control of tomato (Lycopersicum esculentum) post harvest fruit rot.

1*IJATO J.Y, Otoide J.E, 2IJADUNOLA J.A AND ALADEJIMOKUN A.O

1Department of Plant Science, Faculty of Science. University of Ado–Ekiti, P.M.B 5363, Ekiti State, Nigeria.

jamesyeni@yahoo.com

2Federal College of Agriculture, Institute of 2Agricultural Research and Training, Moor Plantation, Ibadan, Nigeria.

3Department of Science Laboratory Technology, School of Science, Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria.

Abstract: The antimicrobial effects of extract of Venonia amygdalina and Tridax procumbens were determined on rot causing fungi. In the present study, the pathogenic fungi isolated from the infected tomato fruit parts and identified based on morphological and cultural characters were: Aspergillus niger, Rhizopus stolonifer, Fusarium oxysporum, Geotrichum candidium. Two different extractive solvents (water and ethanol) were used; aqueous concentrations of 80 and 60% as well as ethanol concentration of 30 and 20% were used in this study. All the plant extracts, both aqueous and ethanol showed significant reduction of mycelia growth of isolated pathogens. In aqueous extract, 80% of both Venonia amygdalena and Tridax procumbens had high inhibitory effect of 49.20% against Geotrichum candidium and 53.30% against Aspergillus niger respectively than 60% aqueous concentration of the test plant extracts, while in ethanolic extract, 30% ethanol extract of Venonia amygdalena and Tridax procumbens inhibited up to 65.20 and72.20% against Fusarium oxysporum respectively more than 20% ethanolic extracts. Higher concentration of both aqueous and ethanol favoured higher mycelia growth reduction. Plant extracts are accessible for controlling phyto diseases, non hazardous, eco-friendly, low cost and non-pollutant.

[IJATO J.Y, Otoide J.E, IJADUNOLA J.A AND ALADEJIMOKUN A.O. Efficacy of antimicrobial effect of Venonia amygdalina and Tridax procumbens in in vitro control of tomato (Lycopersicum esculentum) post harvest fruit rot. Report and Opinion 2011;3(1):120-123]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.15

Key word: Venonia amygdalena, tomato fruit rot, Tridax procumbens and biological control

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Biodegradation of Glyphosate Pesticide by Bacteria isolated from Agricultural Soil

 

*1Olawale, Adetunji, Kolawole and 2Akintobi, Akinsoji, Olubiyi

1Department of Applied Sciences, Osun State Polytechnic, Iree. Nigeria.

2Department of Biochemistry and Microbiology, Lead City University, Ibadan. Nigeria.

E-mail: akolawale@yahoo.com

 

Abstract: Three bacteria strains GDP1, GDP2 and GDA were isolated from agricultural soil heavily polluted with glyphosate which are capable of degrading glyphosate pesticide (100 ppm). The bacteria strains were identified through cultural and biochemical characterization as Pseudomonas putida, P. aeruginosa and Acetobacter faecalis respectively. Degradation of glyphosate by GDP1, GDP2, GDA and a mixed consortium of the three isolates designated CGD were confirmed by solid-phase extraction SPE-LC-ESI-MS assays. Glyphosate-mineralizing populations were determined in a most probable number (MPN) technique. Cell growth levels off at approximately 72hr, which coincides with glyphosate concentration decreasing to zero level.. Of three isolated bacteria GDP1 completes degradation of 50-ugml-1 glyphosate in 20 ml of an enrichment medium BMA at approximately 72hrs faster than GDP2 and GDA which completed at approximately 96hrs. Addition of a mixed consortium of GDP1, GDP2 and GDA did not result in significantly faster degradation processes but reduced the lag times to approximately 12hrs from 24hrs and completes degradation at approximately 72hrs. The developed consortium is potent glyphosate degrader with quick action as indicated by the shorten lag times, and it can be used to remediate soil contaminated with pesticide.

[Olawale, Adetunji, Kolawole and Akintobi, Akinsoji, Olubiyi. Biodegradation of Glyphosate Pesticide by Bacteria isolated from Agricultural Soil. Report and Opinion 2011;3(1):124-128]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.16

Keywords: Degradation, pesticide, glyphosate, Pseudomonas sp., Acetobacter sp

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Mother-To-Child Transfer Of Measles Antibody Among Patients Attending University Of Maiduguri Teaching Hospital, Borno State, Nigeria.

1Haruna S.B, 1 BukBuk D.N, 1Dawurung J.S.

1Department of Microbiology, University of Maiduguri, Nigeria

dawurungj@yahoo.com

ABSTRACT: The transfer of Measles specific IgG antibody was assessed in 128 sera of infants and 30 sera of mother-child pairs at delivery. The 158 infants were within the age range of birth to 9 months. Of the 30 serum samples of mother to child pairs, 30(100%) of the mothers had the antibody but only 28(93.3%) passed the antibody to their newborn which means 2(6.7%) of the infants did not acquire the antibody. In the 158 serum samples of infants tested 44.8% (71) were seropositive which means they acquired the antibody while 55.1% (87) were susceptible to measles virus. The result indicates that with increase in age, the percentage susceptibility of infant increases. The antibody level is high (77.8%) in one day olds and none (0%) in 9 months infants. This shows that infants at late ages before vaccination are susceptible to the measles virus. In conclusion, the result shows that infants within the age of 0 to 3 months have a higher level of antibody than other ages. Hence, susceptibility to measles virus in infants within the ages of 7 to 9 months is high.

[Haruna S.B, BukBuk D.N, Dawurung J.S. Mother-To-Child Transfer Of Measles Antibody Among Patients Attending University Of Maiduguri Teaching Hospital, Borno State, Nigeria. Report and Opinion 2011;3(1):129-133]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.17

Keywords: Mother- to- Child, Transfer, measles, antibody

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Indexing soil P to recommend for durum wheat in East Shewa, Oromiya Region

 

Mesfin Kebede, and Tekalign Tadesse

 

Ethiopian Institute of Agricultural Research (EIAR), Debre Zeit Agricultural Research Center, P. O. Box 32, Debre Zeit, Ethiopia

ethiosoils@gmail.com,and tekalignt@fastmail.fm

 

Abstract: On farm soil test based phosphorous determination research experiment was carried out in three locations of East Showa from 2003-05 cropping seasons. Six levels of P were applied as a treatment (Triple Super Phosphate) and recommended level of nitrogen from urea was applied uniformly to all plots. Application of 15.1 kg P per ha in Ude and 25.1 kg P in Akaki provided the maximum mean grain yields of 3.75 and 1.79 t ha-1, respectively for year (2003). In the 2004 cropping seasons, however, the absence of any application of external P gave the highest (3.38 t ha-1) in Chefe Donsa and the least mean grain yield (1.74 t ha-1) was recorded in Ude by 15.1 kg P . In the year 2005 result revealed that the two treatments from Chefe Donsa and Akaki gave the maximum comparable mean grain yields (4.89 and 4.49 t ha-1) but and the least yield was obtained through the application of soil P only (1.88t) in Ude. For these cropping seasons, a total of twenty seven regression equations were developed and eighteen were selected in order to map the soil P (ppm) versus treatments. Accordingly, an average of 97.0, 96.5 and 96.5 (2003), 86.0, 93.0, and 89.5 (2004) and 92.0, 91.5, and 92.5% (2005) remained in the soil for every application of external P in Akaki, Chefe Donsa and Ude, respectively. In most of the years, on average of over 85% of the applied P remained in the soil. In some locations, non-significant differences were due to this. It seems that our soils are saturated by the application of P years around and becoming the least limiting plant nutrient. Therefore, any phosphorus recommendation for optimum durum wheat production should be on the basis of its soil test P value. This experiment also proved that even if the soil P increases through the application of external P from 6.5ppm onwards, it didn’t reflect in the increment rather the decline of mean grain yield. But further research work has to be conducted to determine the limiting plant nutrient, its index in different soil types, environment and in its interaction.

[Mesfin Kebede, and Tekalign Tadesse. Indexing soil P to recommend for durum wheat in East Shewa, Oromiya Region. Report and Opinion 2011;3(1):134-140]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.18

 

Key Words: Equation, On farm, Soil Test, TSP, Soil Types, Regression

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Corrosion Co-inhibition of Sodium Tungstate with Sodium Nitrate and Sodium Silicate on Low Carbon Steel in HCl

 

1Ogundare, O., 2*Umoru, L.E. and 2Ige, 0.0.

1Dept of Research & Development,

Engineering Materials Development Institute, Akure

2Department of Materials Science & Engg, Obafemi Awolowo University, Ile – Ife

suppiedee@yahoo.com; lumoru@oauife.edu.ng; ige4usa@yahoo.com

Abstract: This study has investigated the co-inhibitive potentials of sodium tungstate with sodium nitrate and sodium silicate on low carbon steel immersed in 0.085 M hydrochloric acid. This was done with a view to determining which of the oxidizing inhibitors will give better co-inhibition efficiency (IE) with sodium tungstate. The study was carried out using the gravimetric technique. The uninhibited solution of 0.085 M hydrochloric acid served as the control. The results obtained have shown that 71% IE was produced by co-inhibiting sodium tungstate with sodium silicate and 54% IE produced by co-inhibiting with sodium nitrate after 336 hours of exposure. This was due to a greater oxidizing condition produced by the former. Sodium tungstate alone gave 47% IE and sodium nitrate produced 57% IE when used alone, and sodium silicate showed 18%IE after 336 hours of exposure.

[Ogundare, O., Umoru, L.E. and Ige, 0.0. Corrosion Co-inhibition of Sodium Tungstate with Sodium Nitrate and Sodium Silicate on Low Carbon Steel in HCl. Report and Opinion 2011;3(1):141-150]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.19

Keywords: Corrosion, Inhibition Efficiency, Co-Inhibitors and Pickling

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Diversity of Potential lichens on Banj oak twigs in Banlekh forest of district Champawat, Kumaun Himalaya

 

Balwant Kumar, *Lalit M. Tewari and Hemlata Kholia

Department of Botany, D.S.B. Campus, Kumaun University, Nainital 263001

* corresponding author email: l_tewari@rediffmail.com

 

Abstract: The Parmelioid lichens on Banlekh forest of Champawat (Uttarakhand) comprises 45-60%, Usnioid (Usneaceae 17-30% and Ramalinaceae 15-30%) found on different diameter class fallen twigs of Quercus leucotrichophora A. Camus (Banj oak). The study observed the twig diameter class 0-1 cm and 1.1-2 cm is excellent for the large thallus of the members of family Ramalinaceae. The villagers of the Banlekh area are not using these 15 potential lichens due to lack of information and knowledge.

[Balwant Kumar, Lalit M. Tewari and Hemlata Kholia. Diversity of Potential lichens on Banj oak twigs in Banlekh forest of district Champawat, Kumaun Himalaya. Report and Opinion 2011;3(1):151-154]. (ISSN: 1553-9873). http://www.sciencepub.net.

doi:10.7537/marsroj030111.20

 

Key words: Lichen, oak twigs, livelihood, Champawat (Kumaun)

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Chromosomal Aberrations Induced in Root Tips of Allium cepa by Squeezed Garri Extracts

 

Daniel I. Olorunfemi and Emmanuel O. Ehwre

Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Benin, Edo State, Nigeria. danfem@yahoo.com, princeemray@yahoo.com

 

Abstract: The potential genotoxic effects of squeezed extracts from toasted cassava granules popularly known as garri, a popular Nigerian cassava meal, obtained from different fermentation days were investigated using the Allium cepa assay. The squeezed extracts were prepared with potable water as practiced conventionally by soaking 1-day fermented, 2-day fermented, 3-day fermented and 4-day fermented garri and squeezing out the liquid. A series of 5 onion bulbs were exposed to 1, 2.5, 5 and 10% (effluents, v/v) concentrations of each of the extracts for macroscopic and microscopic analyses. There was fermentation and concentration-dependent and statistically significant (P < 0.05) inhibition of root growth by the extracts when compared with the control. The EC50 obtained for 1 day, 2 day, 3 day and 4 day fermented toasted cassava granules were 2.5, 2.8, 3.1 and 4.0 respectively. All the tested extracts were observed to have mitodepressive effects on cell division in the increasing order 1-day > 2-day > 3-day > 4-day fermented garri extracts. The results further go to confirm findings from other studies that proper fermentation aids in the reduction of toxic cyanogenic components present in poorly processed cassava products including garri.

[Daniel I. Olorunfemi and Emmanuel O. Ehwre. Chromosomal Aberrations Induced in Root Tips of Allium cepa by Squeezed Garri Extracts. Report and Opinion 2010;3(1):155-160]. (ISSN: 1553-9873).

doi:10.7537/marsroj030111.21

 

Key words: garri, genotoxicity; mitotic index; cyanide; Allium cepa

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Reproductive Performance And Economic Efficiency Of Finn And Rahmani Ewes And Their Crosses

 

Gaafar, H.M.A.; M.T. Shehab El-DIN and M.E. El-Gendy

Agricultural Research Center, Giza, Egypt

dr.gaafar@hotmail.com

 

ABSTRACT: One hundred and fifty Finn (F) and Rahmani (R) ewes and their crosses including 10 F, 60 R, 50 1/4F 3/4R, 20 1/2F 1/2R and 10 3/4F 1/4R were allotted to fed traditional forage diet (D1) contained concentrate mixture + wheat straw plus fresh berseem, (winter diet) or plus berseem hay and green sorghum (summer diet) or agricultural by-product diet (D2) contained concentrate mixture plus fresh berseem, fresh sugar beet tops or green reed plants (winter diet) or plus dried sugar beet tops or green or dried reed plants (summer diet). Results showed that 81% of ewes were mating during the period from April to August reaching the maximum in July. However, 83% of ewes were lambing from October to April reaching maximum in December. The fertility expressed as ewe lambing per ewe exposed (EL/EE) was higher in crossbred that pure Finn and Rahmani ewes and increased with increasing Rahmani blood. The number of lamb born and weaned per ewe exposed (LB/EE and LW/EE) was higher in the first ewes crossbred of 1/2F 1/2R than the pure breeds and other crosses. Pure Finn recorded the highest number of lamb born and weaned per ewe lambing (LB/EL and LW/EL), lambing ewe per year (EL/Y) and lamb born and weaned per lambing ewe per year (LB/EL/Y and LW/EL/Y), but Rahmani had the lowest values and increased in crossbred ewes with increasing Finn blood. Pure Rahmani breed showed the lowest FC/E/Y and 1/4 F 3/4 R had the higher OLW/E/Y and NR/E/Y, however Finn breed had the highest FC/E/Y and the lowest OLW/E/Y and NR/E/Y. Moreover, the FC/E/Y increased (P<0.05) and OLW/E/Y and NR/E/Y decreased (P<0.05) with increasing the percent of Finn blood in crossbred.

[Gaafar, H.M.A.; M.T. Shehab El-DIN and M.E. El-Gendy. Reproductive Performance And Economic Efficiency Of Finn And Rahmani Ewes And Their Crosses. Report and Opinion 2010;3(1):161-165]. (ISSN: 1553-9873).

doi:10.7537/marsroj030111.22

 

Keywords: Ewes, Finn, Rahmani, crossbred, feeding, reproductive, economic efficiency

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The manuscripts in this issue were presented as online first for peer-review, starting from November 8, 2010. 
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