Report and Opinion
5 - Issue 10, Cumulated 52, October 25, 2013, ISSN 1553-9873
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Titles / Authors
The development of PCR
test-systems for diagnostics of virus diseases
Assilbek Burabaev1, Valentina Matveeva2,
Zhumagaly Koshemetov2, Marina Koryagina2,
South Kazakhstan State Pharmaceutical Academy, Shymkent,
Republic of Kazakhstan
Research Institute for Biological Safety Problems, Almaty,
Republic of Kazakhstan
The results of the data obtained on the basis of the development
of diagnostic test- system RT-PCR based on the hemagglutinin
gene F / R for identifying equine influenza virus of strain
H3N8, showed high sensitivity and specificity in working
conditions. And RNA can be reliably detected in the researched
virus strains. Viral fragment 200 b.p. was shown as the product
in the result of the amplification, with a sensitivity of 10–9TCD50/ml
and the high specificity of the reaction. Isolated RNA maintains
its activity for 2-10 months at -18 º C. Research work on the development of diagnostic test- system for
the detection of HA gene, the gene of EIV, was hold on the basis
of RSE RIBSP. In connection therewith, we are announcing our
gratitude to all members of the laboratory of diagnostic and
indication of viral infections.
[Assilbek Burabaev, Valentina Matveeva, Zhumagaly Koshemetov,
Marina Koryagina, Marlen Yessirkepov, Bekaidar Nurmashev. The
development of PCR test-systems for diagnostics of virus
diseases. Rep Opinion
2013;5(10):1-6]. (ISSN: 1553-9873).
PCR test-systems, diagnostics, virus diseases
Hangul (Cervus elaphus hanglu) depletion in Dachigam
National Park and its adjoining mountainous ranges in Kashmir
valley: A Review
Nasir Rashid Wani
Faculty of Forestry, Sher-e-Kashmir University of Agricultural
Sciences and Technology of Kashmir, Shalimar (J & K) 191121,
The Kashmir Red Deer or Hangul (Cervus elaphus hanglu) a
member of family cervidae being the state animal is presently
surviving only under the moist temperate forests of Kashmir
region. Most of its population is concentrated in the world
famous Dachigam National Park which covers an area of 141 square
kilometers and is located on foothills of Zabarwan range among
the high mountains of the mighty western Himalayas. In the past
Kashmir had a large and vibrant population of Hangul.Now at
present the Hangul has been declared critically endangered
species by Red Data Book of the International Union for
Conservation of Nature and Natural Resources (IUCN). The
declining population of Hangul in Kashmir is because of the
shrinkage of forests due to deforestation, fragmentation of
natural habitat due to closure of corridor links between Overa
Wildlife Sanctuary and Shikargah Conservation Reserve as well as
other potential habitats, contamination of high altitude water
bodies due to dam projects and climatic changes due to tourism
and recreation and also high population pressure has
significantly curbed the wild population of Hangul.There is an
urgent requirement to initiate a conservation breeding programme
to augment Hangul population in the wild.
Rashid Wani. Hangul (Cervus elaphus hanglu)
depletion in Dachigam National Park and its adjoining
mountainous ranges in Kashmir valley: A Review.
Dachigam, Hangul, population depletion, physical factors,
The Clinicopathological Characteristics of Uterine Leiomyomas: A
Tertiary Care Centre Experience in Saudi Arabia
Uterine leiomyoma in Saudi Arabia
Abdullah1, Wafaey Gomaa1,2
of Pathology, Faculty of Medicine, King Abdulaziz University,
Jeddah, Saudi Arabia
of Pathology, Faculty of Medicine, Minia University, El-Minia,
Leiomyomas are very common tumours of the female genital tract.
However, large cohort studies are limited for reporting the
incidence and prevalence. The aim of this study is to review the
clinical and pathological pattern of leiomyoma in a tertiary
care centre in Saudi Arabia. Methods: We retrospectively
analysed the clinical and pathological data of patients
diagnosed as leiomyoma in a large tertiary care centre in Saudi
Arabia in the period from 2005 to 2012. Results: A total
number of 563 patients were diagnosed as uterine leiomyoma. The
age range was 22-86 years (mean 41.8 years). Leiomyomas were
more common in age groups 41-50 years (40.1%) (p<0.001)
followed by the age group 31-40 years (35.2%). Dysfunctional
uterine bleeding was the presenting symptom in 19.2% followed by
pelviabdominal mass (4.6%). In 70 % of cases, the specimen sent
was delivered as myomectomy while in 30% leiomyoma was diagnosed
in a hysterectomy specimen. The majority of leiomyomas were
ordinary leiomyoma (97.3%) (p<0.001). Cellular leiomyoma
constituted 2.7%. Hyaline degeneration was the commonest
associated histological change (5.5%) followed by infarction
(2.1%), and calcification (0.4%). The vast majority of
leiomyomas were located intramural and multiple leiomyomas were
significantly higher than single leiomyomas (p<0.001).
Discussion: The present study is consistent with previously
published data and confirms that leiomyomas are common benign
neoplasms of females especially in age group of 31-50 years
which may cause considerable morbidity. Further research and
increase awareness on this tumour is required.
[Layla Abdullah, Wafaey
Gomaa. The Clinicopathological Characteristics of
Uterine Leiomyomas: A Tertiary Care Centre Experience in Saudi
Arabia. Rep Opinion
2013;5(10):21-26]. (ISSN: 1553-9873).
words: Leiomyoma, Fibroid, Pathology, Saudi Arabia.
A Review Of
Effects Of Desertification On Food Security
Nneji, Lotanna Micah
Department of Biological
Sciences, University of Abuja, PMB 117, Abuja, Nigeria
Desertification is the process of destruction of the biological
potential of land that can ultimately lead to desert-like
conditions due to widespread deterioration of ecosystems under
the combined pressure of adverse and fluctuating climate and
excessive exploitation of the land.
It is an advanced stage of land degradation where soil has lost
part of its capability to support human communities and
ecosystem. The main causes of desertification are climatic
variation (drought) and human activities which include extensive
agriculture, deforestation, overgrazing and urbanization.
Desertification can results to land degradation leading to
decreased productivity of the arable land, resulting to a
serious threat to the environment, agricultural sustainability
and human welfare.
It is directly linked to deterioration of soil health which in
turn affects crop productivity and sustainability, causing food
insecurity. Reduction in food availability, use and
accessibility can be attributed to the deleterious effects of
for combating desertification include
planting of trees, landscape restoration, application of
fertilizers on the soil etc. Furthermore, International
Organization and governments at national, state and local level
should enlighten the general public on the effects of
desertification on food security.
A Review of the Effects of Desertification on Food Security.
Rep Opinion 2013;5(10):27-33].
Keywords: Desertification, food security, agriculture, drought,
deforestation, overgrazing, urbanization
CIN In Upper Egypt
Edessy M.S *, Aldarwish A *,
Hala Naguib Hosni **, Galal M **, and Ali F ***.
Professor*, lecturer*** of
Obstetrics and Gynecology Al Azhar University and Ass professor
of pathology Cairo university**
The present study was To evaluate
the effectiveness and safety of the loop electrosurgical
excision procedure (LEEP) in the treatment of cervical
intraepithelial neoplasia (CIN), Women participating in a
cervical screening study with histologically confirmed cervix
were visually inspected with acetic acid, followed by
coloposcopy and biopsy taken from abnormal areas, Cure was
defined as no clinical or histologic evidence of CIN. Factors
influencing cure rates were evaluated by χ2 tests Out
of the 1000 screened women 126 were found to be CIN positive
(12.6%), 120 underwent LEEP. Six months follow up of 114 cases
showed complete cure of 108 women
(94.7%), LEEP Cure rates were 96.7%, 88.9%, and 80% for
CINI, CINII and CINIII
respectively. Single Pass and Multiple Pass cure rates were 97%
and 90% respectively. Minor adverse effects were observed in 15
women and complications were seen in 5 women, LEEP was
associated with minimal complications and good cure rates
especially in those with CINI
even in cases with large lesions.
M.S, Aldarwish A, Hala Naguib Hosni, Galal M, and Ali F. CIN In Upper Egypt LEEP Excision.
Rep Opinion 2013;5(10):34-40].
effectiveness; safety; loop
electrosurgical excision procedure (LEEP); cervical
intraepithelial neoplasia (CIN); histology; biopsy; lesion
Antimicrobial Evaluation of the Stem Bark Extracts of Parkia
biglobosa (Jacq.) Benth. Ex G. Don
Olubiyi Akinsoji Akintobi1, Adekunle Odunayo Adejuwon2,
Adetunji Kola Olawale3
of Microbiology and Biochemistry, Faculty of Information
Technology and Applied Sciences, Lead City University, Ibadan,
of Applied Sciences, Osun State Polytechnic, Iree, Nigeria
The stem bark extract of Parkia biglobosa (Jacq.) Benth.
Ex G. Don was evaluated for antimicrobial activity. Isolates
tested were pathogenic strains of Pseudomonas aeruginosa,
Escherichia coli, Klebsiella pnuemoniae,
Proteus mirabilis, Aspergillus flavus and
Aspergillus fumigatus. The bark extracts of P. biglobosa
used were of the solvents hexane, ethanol, petroleum ether
and water. The extracts were concentrated at 70oC
using a rotary evaporator and then plated at different
concentrations with the isolates using the agar well diffusion
technique. The ethanolic extract of the stem bark had
antimicrobial effect on all the isolates with a minimum
inhibitory concentration (MIC) of 40 mg/ml for the isolates
except A. flavus and K. pneumonia which were
inhibited the MIC of 66.6 mg/ml. The hexane and petroleum ether
extracts had no effect on any of the pathogens however, the
aqueous extract was slightly active on K. pneumonia at an
undiluted concentration. When evaluated with specific broad
spectrum antibiotics, ciprofloxacin had efficacy on K.
pneumonia, P. mirabilis and P. aeruginosa
comparing favourably with the solvent extracts of P.
[Akintobi OA, Adejuwon AO, Olawale AK. Antimicrobial
Evaluation of the Stem Bark Extracts of Parkia biglobosa
(Jacq.) Benth. Ex G. Don.
2013;5(10):41-45]. (ISSN: 1553-9873).
hexane, ethanol, petroleum ether, aqueous, bacteria, fungi
Analysis Of Energy Balance On
Nigeria’s Economic Growth: Evidence On Electricity Sector:
Amadi, C. R1., Amadi,
N. N2., Anyim, E.3
Of Banking And Finance, Rivers State Polytechnic, Bori, Nigeria
Of Economics, Ignatius Aguru University Of Education, Port
Of Economics, University Of Uyo, Nigeria
This study analysed the effects
of energy balance on Nigeria’s economic growth, with specific
focus on the electricity sector. The endogenous growth model
complimented with an econometrics packages were adopted to
determine the relationship between energy (electricity) demand,
energy supply and energy balance: their stationarity and short
and long run effects. The parsimonious estimate declared the
relevance of electricity supply and demand to economic growth.
To test the impact of electricity balance on economic growth,
the second model included energy (electricity) balance, this
resulted to overall change in influence and significance. The
implication to the study is that the energy difference caused by
excess demand is a strong determinant to the diabetic economic
growth in the country. Against this background, this study
suggests adequate funding, rehabilitation of existing power
plants and construction of new ones to support the existing
ones. Also recommended is the exploitation of nuclear sources of
power supply, intensification of efforts to checkmate vandals
and thieves of power apparatus, distilled massive private
investment and incentives via multi year tariff order (MYTO) and
gas sales agreement that eliminated direct government subsidies.
[Amadi, C. R., Amadi, N. N.,
Anyim, E. Analysis Of Energy Balance On Nigeria’s Economic
Growth: Evidence On Electricity Sector: 1970-2010.
energy balance, Nigeria,
Economic growth, Electricity sector
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