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Science Journal

 

Stem Cell 

ISSN: 1545-4570 (print); ISSN: 1945-4732 (online)

 Volume 5 - Issue 2 (Cumulated No. 18), June 25, 2014; ISSN 1554-0200

Cover Page, Introduction, Contents, Call for Papers, Stem0502

 

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CONTENTS  

No.

Titles / Authors /Abstracts

Full Text

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1

Stem Cell Introduction

 

Ma Hongbao 1, Margaret Ma 2

 

1 School of Life Sciences, Zhengzhou University, Zhengzhou, Henan 450001, China

2  Cambridge, Massachusetts 02138, USA

ma8080@gmail.com

 

Abstract: The stem cell is the origin of an organism’s life that has the potential to develop into many different types of cells in life bodies. In many tissues stem cells serve as a sort of internal repair system, dividing essentially without limit to replenish other cells as long as the person or animal is still alive. When a stem cell divides, each new cell has the potential either to remain a stem cell or become another type of cell with a more specialized function, such as a red blood cell or a brain cell.

[Ma H, Ma M. Stem Cell Introduction. Stem Cell 2014;5(2):1-10] (ISSN 1545-4570). http://www.sciencepub.net/stem. 1

doi:10.7537/marsscj050214.01

 

Keywords: stem cell; origin; organism; life

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2

Totipotent of Stem Cell

 

Ma Hongbao 1, Margaret Ma 2

 

1 School of Life Sciences, Zhengzhou University, Zhengzhou, Henan 450001, China

2 Cambridge, Massachusetts 02138, USA

ma8080@gmail.com

 

Abstract: The definition of stem cell is an unspecialized cell that gives rise to a specific specialized cell, such as a blood cell. Embryonic stem cells are derived from the inner cell mass of blastocyst stage embryos. Somatic stem cells differentiate into only the cells the specific tissue wherein they reside. Stem Cell is the original of life. All cells come from stem cells. The totipotent of stem cell is the most important property for the stem cell to keep the life continuously.

[Ma H, Ma M. Totipotent of Stem Cell. Stem Cell 2014;5(2):11-15] (ISSN 1545-4570). http://www.sciencepub.net/stem. 2

doi:10.7537/marsscj050214.02

 

Key words: DNA; eternal; life; stem cell; universe

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3

Trials for Replacing Inorganic N Partially in Superior Vineyard by Using Slow Release N Fertilizers, Humic Acid and EM

 

Faissal F. Ahmed1; Mohamed A.M. Abada 2; Ali H. Ali 1 and Haitham M. Allam 2

 

1Hort. Dept. Fac. of Agric. Minia Univ. Egypt.

2 Viticulture Dept. Hort. Res. Instit. ARC, Giza, Egypt

Faissalfadel@yahoo.com

 

Abstract: During 2012 and 2013 seasons, Superior grapevines were supplied with N (50 g / vine / year) via 19 to 56% of the three slow release N fertilizers namely sulphur-coated urea, phosphorus-coated urea and urea- formaldehyde besides the check treatment in which the vines fertilized with N as 75% urea the fast release N fertilizer. In addition to the fast and slow release N fertilizers, the vines received humic acid and/ or effective microorganisms (EM) each at 10 ml/ vine/ year. Leaf area, totals chlorophylls, N, P and K, berry setting %, yield, berries quality , juice content of nitrate and nitrite, soil content of CO2 and total counts of bacteria and wood ripening coefficient in response to slow and fast release N fertilizers, humic acid and EM treatments were investigated. Application of N through 19 to 56% any one of the slow release N fertilizers effectively enhanced the leaf area, total chlorophylls , N, P and K in the leaves, berry setting %, yield, berries quality , soil content of CO2 and total counts  of bacteria , while reduced both nitrate and nitrite in the juice relatively to using N as 75% urea. Single and combined applications of humic acid and/ or EM each at 10 ml / vine considerably improved leaf area, vine nutritional status, yield, berries quality and soil content of CO2 and total counts of bacteria. Using EM was superior than using humic acid in this respect. The best results with regard to yield and quality of Superior grapes were obtained with supplying the vines with N (50 g / vine / year) via 38% phosphorus- coated urea + 5 kg farmyard manure + humic acid and EM each at 10 ml/ vine year. Pollution with nitrate and nitrite was greatly reduced with using such promised treatment.

[Faissal F. Ahmed; Mohamed A.M. Abada ; Ali H. Ali  and Haitham M. Allam. Trials for Replacing Inorganic N Partially in Superior Vineyard by Using Slow Release N Fertilizers , Humic Acid and EM. Stem Cell 2014;5(2):16-29] (ISSN 1545-4570). http://www.sciencepub.net/stem. 3

doi:10.7537/marsscj050214.03

 

Keywords: Trials; Inorganic; Vineyard; Slow Humic Acid

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4

The Synergistic Effects of Using Plant Extracts and Salicylic Acid on Yield and Fruit Quality of Keitte Mango Trees

 

Faissal F. Ahmed1; Mohamed Kh. Kamel2 and Hamdy I.M. Ibrahim1

 

1Hort. Dept. Fac. of agric. Minia Univ. Egypt

2Experts of Ministry Justice, Minia

faissalfadel@yahoo.com

 

Abstract: During 2012 and 2013 seasons, Keitte mango trees subjected to foliar application of some plant extracts namely oils of onion and garlic and extracts of green tea and turmeric each at 0.1% and / or salicylic acid at 100 ppm. The study focused on the impact of plant extract and salicylic acid treatments on growth, percentages of healthy and malformed inflorescences, length and six ratio of these inflorescences , plant pigments, N, P, and K in the leaves, fruit setting %, yield and fruit quality. Single and combined applications of onion and garlic oils as well as extracts of green tea and turmeric each at 0.1% and/ or salicylic acid at 100 ppm was very effective in stimulating growth , healthy inflorescences % , plant pigments , nutrients, fruit setting%, yield and fruit quality relatively to the control treatment. Using all plant extracts was favourable than using salicylic acid in this respect. Application of onion oil, garlic oil, green tea extract and turmeric extract, in ascending order had an announced effect on fruiting of the trees. Using each plant extract plus salicylic acid was superior than using each alone in this respect. The best results with regard to yield and fruit quality of Keitte mango trees were obtained due to spraying the trees three times with turmeric extract at 0.1% plus salicylic acid at 100 ppm.

[Faissal F. Ahmed; Mohamed Kh. Kamel and Hamdy I.M. Ibrahim. The Synergistic Effects of Using Plant Extracts and Salicylic Acid on Yield and Fruit Quality of Keitte Mango Trees. Stem Cell 2014;5(2):30-39] (ISSN 1545-4570). http://www.sciencepub.net/stem. 4

doi:10.7537/marsscj050214.04

 

Keywords: Plant extracts, salicylic acid, Keitte mango, yield and fruit quality

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5

Effect of Partial Replacement of Inorganic N Fertilizer Partially By Using EM, Compost Tea and Humic Acid on Fruiting of Sakkoti Date Palms

 

Faissal F. Ahmed1; Ahmed M.M.A. Akl1; Emad A.H. El- Mamlouk2 and Hamdy H.M. Saied

1Hort. Dept. Fac. of Agric. Minia Univ. Egypt

2 Central Lab. For Organic Agric. Giza, Egypt

Hamdi200052005@yahoo.com

 

Abstract: The possibility of replacing 25 to 75% of chemical N fertilizer in Sakkoti date palm orchard partially by using EM, compost tea and humic acid each at 100 to 300 ml/ palm/ year was investigated during 2012 and 2013 seasons. Results revealed that using N via 50 to 75% besides inorganic N + 10% organic N + EM as well as compost tea or humic acid each at 100 to 200 ml/ palm/ year was very effective in stimulating the total surface area per plant, fruit retention%, yield, bunch weight as well as physical and chemical characteristics of all the fruits, relatively to using N via inorganic N form alone at 25 to 100% or when inorganic N was applied at percentages lower than 50% . The promotion was associated with using EM, compost tea and humic acid, in descending order. Using N at 25% inorganic N with or without EM, compost tea or humic acid considerably caused an adverse effects on growth and fruiting of the palms.  The best results with regard to yield and fruit quality of Sakkoti date palms were obtained as a result of using N as 50% inorganic N + 10% organic N (40 kg F.Y.M)+ 200 ml/ EM / palm/ year. Nitrite and nitrate in the pulp were greatly decreased.

[Faissal F. Ahmed; Ahmed M.M.A. Akl; Emad A.H. El- Mamlou and Hamdy H.M. Saied. Effect of Partial Replacement of Inorganic N Fertilizer Partially By Using EM, Compost Tea and Humic Acid on Fruiting of Sakkoti Date Palms. Stem Cell 2014;5(2):40-51] (ISSN 1545-4570). http://www.sciencepub.net/stem. 5

doi:10.7537/marsscj050214.05

 

Keywords: Sakkoti date palms; EM, compost tea, humic acid and productivity.

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6

Insulin producing cell clusters derived from very small embryonic like stem cells are potent to treat diabetics mice

 

Abouzary M, Kashani I , *, Pasbakhsh P, Atlasy N

 

Tehran University of medical sciences, Tehran , Iran

Ragerdi@tums.ac.ir

 

Abstract: Considerable effort has been expended to isolate stem cells from a number of different tissues to use in regenerative medicine. In this study, we purposed to reckon up the ability of mouse very small embryonic like stem cells (VSEL) differentiation into insulin producing cell clusters(IPCCs), in order to treatment of diabetes. Mouse Bone marrow VSEL stem cells, isolated with Fluorescent activating cell sorting (FACS) method by using Fluorescent antibodies against CD45, CXCR4 and Sca1markers. After differentiation of VSEL stem cells into IPCCs with Activin A and nicotinamide as main factors of differentiation, RT-PCR and immunocytochemistry revealed that newly IPCCs could express Ins1, Ins2, PDX1 and Glut2. Invitro insulin secretion by IPCCs was confirmed by ELISA kit. For treatment, we injected IPCCs into thigh muscle of diabetic mice. Results showed that newly derived IPCCs were capable to produce insulin in vivo. There was a significant decrease in mean blood sugar of diabetic mice after passing 3 weeks from cell therapy. This study provides a strategy for using autologous VSEL stem cells for curing diabetes and other regenerative disease, as an alternative for other stem cell types.

[Abouzary M, Kashani I, Pasbakhsh P, Atlasy N. Insulin producing cell clusters derived from very small embryonic like stem cells are potent to treat diabetics mice. Stem Cell 2014;5(2):52-63] (ISSN 1545-4570). http://www.sciencepub.net/stem. 6

doi:10.7537/marsscj050214.06

 

 Key words: Diabetes 1, Very small embryonic like stem cells (VSEL), Fluorescent activating cell sorting (FACS) method, Insulin Producing cell clusters (IPCCs).

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7

Hematological Significance of Vitamin A, C and E Bi-combination in Pregnancy

 

Iribhogbe O. I1, Emordi J.E1, Akpamu U2, Nwoke E.O2, Aigbiremolen A1

 

Department of 1Pharmacology and Therapeutics; 2Physiology; College of Medicine Ambrose Alli University Ekpoma, Edo State Nigeria

Email: oignis@yahoo.com; Mobile Phone No: +2348065794437

 

ABSTRACT: The present study evaluates the correlation between antioxidant vitamins A, C and E bi-combinations and basic hematological parameters in pregnancy. 70 adult female Wister rats weighing between 250- 300g were procured and grouped into 2 control groups treated with distilled H2O and vehicle tween-80 respectively and three cohorts (I, II and III) with four sub-groups (n = 5). After mating with male Wister rats, pregnancy was confirmed on the 6th day. Administration of test agents began on the 7th day. Cohort group I received a combination of vitamin A and C, group II; vitamin A and E and group III; vitamin C and E respectively at four different doses for 11 days. Blood samples were collected 24hrs after completion of treatment and analyzed for Hemoglobin concentration (Hb), white blood cell (WBC) and differential count.  Results revealed a significant increase (p<0.05) in Hemoglobin concentration in the vitamin A, C and E bi- combination treated groups. This increase was dose dependent. WBC count was significantly lower (p<0.05) in experimental groups as compared to the control (8300.00 836.66/mm3). However, increase in dose brought about increase in WBC count in the experimental groups. Polymorph and lymphocyte count presented mixed results compared to control values following alteration in dosage. Antioxidant vitamins A, C and E bi-combinations posses synergetic potentials and appear promising in pregnancy.

[Iribhogbe O. I, Emordi J.E, Akpamu U, Nwoke E.O, Aigbiremolen A. Hematological Significance of Vitamin A, C and E Bi-combination in Pregnancy. Stem Cell 2014;5(2):64-70] (ISSN 1545-4570). http://www.sciencepub.net/stem. 7

doi:10.7537/marsscj050214.07

 

Key wordsHematological parameters, Antioxidant Vitamins, Bi- combination therapy

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8

Anthocyanin content in different parts of capparis spinosa growing wild in Tafresh/Iran

 

Moghaddasian, Behnaz*, Eradatmand Asli Davood and Eghdami Anoosh

 

Department of Horticulture, Faculty of Agriculture, Saveh Branch, Islamic Azad University, Saveh, Iran

 

Abstract: Capparis spinosa has been introduced as a specialized culture in some countries for its anthioxidant properties during the last four decades..It contains a number of chemically active and diverse secondry metabolities, in particular, anthocyanin. In this study the content of anthocyanin was quantitatively determined in different plant parts of C.spinosa at the full flowering stage using spectrophotometric analysis. Collection of plant material was made from Tafresh, Iran Plant were separated into root,stem, leaves, flower bud, full flower, fresh fruit which were dried separately, and subsequently assayed for total anthocyanin contents.The content of anthocyanin varied from 74 mg/100g to 266 mg/100g Among different parts of caper. Flower, leaves and fruit had higher content of anthocyanin respectively. The significant amounts of these antioxidants confirm the nutritional and medicinal value of caper.

[Moghaddasian, Behnaz, Eradatmand Asli Davood and Eghdami Anoosh. Anthocyanin content in different parts of capparis spinosa growing wild in Tafresh/Iran. Stem Cell 2014;5(2):71-74] (ISSN 1545-4570). http://www.sciencepub.net/stem. 8

doi:10.7537/marsscj050214.08

 

Key words: Caper, anthocyanin, medicinal use, nutritional value

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9

Reverse of Life, Immortality and Stem Cell

 

Ma Hongbao 1, Margaret Ma 2, Mary Young 3

 

1 School of Life Sciences, Zhengzhou University, Zhengzhou, Henan 450001, China

2 Cambridge, Massachusetts 02138, USA

3 Brooklyn, New York 11212, USA

ma8080@gmail.com

 

Abstract: In the universe, all the results come from the reasons, and all the reasons create results. In the timeline of the universe, all the current events (or universe condition) come from the past and become the future. Under a certain determined condition, all the factors exist. As the reason, it creates new condition. The younger condition of a life body changes to old under certain condition. Under other condition, the life body could change to the younger. Normally, the adult animal cells come from the differentiation of stem cell. However, there is another change event of cells called transdifferention. By the transdifferention, mature somatic cells transform into other mature somatic cells or even stem cell, which means the life body changes to older to younger. The adult cells are old cells and stem cells are younger. Everything is possible under a certain condition. The universe, the time, space and matter – all are unlimited and the possibility is unlimited also. The life is an existing of physical and chemical existence. Everything is possible, including the reverse of the life processing.

[Ma H, Ma M, Young, M. Reverse of Life, Immortality and Stem Cell. Stem Cell 2014;5(2):75-79] (ISSN 1545-4570). http://www.sciencepub.net/stem. 9

doi:10.7537/marsscj050214.09

 

Key words: DNA; eternal; life; stem cell; universe

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10

Immune response following the administration of the anabolic steroid Boldenone Undecylenate in rabbits.

 

Nahed Saleh, Emad Waded

 

Department of Clinical Pathology, Faculty of Veterinary Medicine, Menufiya University, El-Sadat Branch, Egypt.

nahedsaleh2001@yahoo.com

 

Abstract: The present study was conducted to clarify the effect of the anabolic steroid boldenone undecylenate (BOL) on some immunological parameters in rabbits. The study was carried out on 60 apparently healthy New Zealand white male rabbits. Rabbits were divided into three groups each of 20 rabbits. The first group was injected with an adjuvant substance (sesame oil) and was considered as control (C). The second group was administered BOL at the recommended dose for rabbits (4.5mg/kg b.w) (group R). The third group was administered BOL at a dose double the recommended dose (9 mg/kg b.w) (group D). All treatments were given three times at three weeks interval. Blood samples were collected three times after the last drug injection at two weeks interval. Evaluated parameters included growth performance parameters [total weight gain (TWG), feed conversion rate (FCR) and feed efficiency (FE)], evaluation of cell-mediated immunity [total (TLC) and differential leukocytic counts (DLC), phagocytic activity of heterophils (PA) and phagocytic index (PI)], and evaluation of humoral immune response [antibody titers by hemagglutination inhibition test (HIT), serum levels of IgA and IgG, in addition serum protein electrophoretic fractionation profile]. The results showed that BOL injection into rabbits resulted in a significant increase in TWG and FE and a significant decrease in FCR particularly with the double dose of the drug. Monitoring the parameters of cell mediated response revealed a significant decrease in lymphocyte percentage, PA and PI. In regard to humoral immunity, the results demonstrated a significant decrease in serum antibody titers measured by HIT, gamma globulins as well as serum concentrations of both IgA and IgG. In most cases these alterations in the immune responses were more significant when the double dose of BOL was administered. This study concludes that administration of BOL to rabbits although, results in a significant improvement in growth rate, it compromises the immune response inhibiting both cell mediated and humoral immune reactions. Nevertheless, the results suggest that the severity of these immunological side effects is dose-dependent.

[Nahed Saleh, Emad Waded. Immune response following the administration of the anabolic steroid Boldenone Undecylenate in rabbits. Stem Cell 2014;5(2):80-87] (ISSN 1545-4570). http://www.sciencepub.net/stem. 10

doi:10.7537/marsscj050214.10

 

Keywords: boldenone undecylenate; rabbits; immune response; anabolic steroids

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The manuscripts in this issue were presented as online first for peer-review, starting from June 15, 2014. 
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